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plv teto tfap2c addgene  (Addgene inc)


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    Structured Review

    Addgene inc plv teto tfap2c addgene
    Plv Teto Tfap2c Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plv+teto+tfap2c/pMD2%2EG+(Plasmid+%2312259)/pm41233591-385-117-118
    Average 98 stars, based on 14890 article reviews
    plv teto tfap2c addgene - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), pLX_311-KRAB-dCas9 (Addgene, #96918) and pXPR_050 (Addgene, #96925) were obtained from Addgene. ..

    Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and pLV-tetO-Tfap2c (Addgene, #70269) were obtained from Addgene. .. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani.

    Clone Assay:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), pLX_311-KRAB-dCas9 (Addgene, #96918) and pXPR_050 (Addgene, #96925) were obtained from Addgene. ..

    Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and pLV-tetO-Tfap2c (Addgene, #70269) were obtained from Addgene. .. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani.

    Sequencing:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), pLX_311-KRAB-dCas9 (Addgene, #96918) and pXPR_050 (Addgene, #96925) were obtained from Addgene. ..

    Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells
    Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and pLV-tetO-Tfap2c (Addgene, #70269) were obtained from Addgene. .. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani.



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    Addgene inc tfap2c knockout cell lines
    Fig. 4. Genetic depletion of <t>TFAP2C</t> in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.
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    Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

    Journal: Scientific reports

    Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

    doi: 10.1038/s41598-025-03830-x

    Figure Lengend Snippet: Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

    Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

    Techniques: Western Blot, Knock-Out, Clone Assay, Control, Expressing, Immunofluorescence

    Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

    Journal: Scientific reports

    Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

    doi: 10.1038/s41598-025-03830-x

    Figure Lengend Snippet: Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

    Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

    Techniques: