plv teto tfap2c addgene (Addgene inc)
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Plv Teto Tfap2c Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 14890 article reviews
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Plasmid Preparation:Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and Clone Assay:Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and Sequencing:Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), Article Title: Scalable and Efficient Generation of Mouse Primordial Germ Cell-like Cells Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, # 139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259) and |
